human foreskin fibroblast cells  (ATCC)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: Results of DNA assay used to determine the attachment of (A) BJ5ta cells and (B) MLOA5 cells onto PCL-only, PGS-M-only, and PCL-M:PGS-M porous scaffolds after 24 h compared to tissue culture plate monolayer (N = 3, n = 3, *P < 0.05 and ***P < 0.001). Results of resazurin assay from (C) BJ5ta cells and (D) MLOA5 cells seeded onto PCL-M-only, PGS-M-only, and PCL-M:PGS-M porous scaffolds on day 1, 4, and 8 (N = 3, n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001). Representative water contact angle images of (E) bulk PCL-M, (F) bulk PGS-M, and (G) bulk 50:50 PCL-M:PGS-M.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Resazurin Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: (A) Comparison of Sirius red staining (SRS) on PCL-M:PGS-M scaffolds seeded with BJ5ta cells and MLOA5 cells vs. acellular scaffolds after 14 days of culture. (B) SRS absorbance readings on scaffolds vs. on tissue culture plastic (TCP). (N = 3, n = 3; *P < 0.05, **P < 0.01). For all readings, the background colour (acellular controls) was subtracted.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Comparison, Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: Fluorescent staining (blue: DAPI, green: Phalloidin FITC) of (A) BJ5ta cells and (B) MLOA5 cells on day 4 of culture on porous PCL-M:PGS-M scaffolds. BJ5ta cells were observed to grow into the largest pores of the scaffold as shown by (C) the pore outline and (D) the depth of the cells within it.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: H&E staining of scaffold cross sections shows infiltration of BJ5ta and MLOA5 cells into PCL-M:PGS-M, PGS-M-only, and PCL-M-only polyHIPE scaffolds after 7 days of culture. Scale bars represent 250 μm. Magnification is ×10. Top of scaffolds (cell seeded surface) is indicated on images.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Staining

Journal: bioRxiv

Article Title: Eukaryotic Initiation Factor 5B (eIF5B)-Driven Translational Control Impacts Oral Squamous Cell Carcinoma Pathophysiology

doi: 10.64898/2026.01.30.702967

Figure Lengend Snippet: BJ-5ta cells were transfected with either a non-specific control siRNA (siC) or an eIF5B-specific siRNA pool (si-eIF5B). (A) Following 24 h of transfection, cells were treated with either vehicle control or TRAIL (100 ng/mL). Cell viability was assessed after an additional 72 h using alamarBlue TM assays. (B) Annexin V and 7-AAD staining and flow cytometric analysis of transfected cells. The top two panels, siC and siC + TRAIL, the bottom two panels, si5B and si5B + TRAIL, were harvested at 96 h post-transfection and treated with Annexin V + 7AAD. Contour plots are divided into quadrants Live (lower left; cells staining negative for both 7-AAD and annexin V), Q1 (upper left; cells staining positive for 7-AAD but negative for annexin V), Early apoptosis (lower right; staining annexin V positive and 7-AAD negative), and Late apoptosis (upper right; staining positive for both 7-AAD and annexin V). (C) Representative immunoblots probing for eIF5B, XIAP, Bcl-xL, cIAP1, cFLIPs, DR 4, DR 5, and β-actin (internal control) are shown. ( D) Control and eIF5B-depleted BJ-5ta cells were treated with the 4EGI-1 inhibitor to inhibit cap-dependent translation. Levels of XIAP were monitored under control and the 4EGI-1 treatment conditions. (E) eIF5B-bound RNA immunoprecipitations (RIPs) followed by qRT-PCR measurements of input-normalized tRNAiMet levels from BJ-5ta vs UMSCC-29. Data are presented as mean ± SEM from three independent biological replicates, with statistical significance indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: CAL-33 and BJ-5ta (immortalized fibroblast) cell lines were obtained from American Type Culture Collection (ATCC; Virginia, USA), and UMSCC-1 and UMSCC-29 were obtained from the University of Michigan (Michigan, USA).

Techniques: Transfection, Control, Staining, Western Blot, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Eukaryotic Initiation Factor 5B (eIF5B)-Driven Translational Control Impacts Oral Squamous Cell Carcinoma Pathophysiology

doi: 10.64898/2026.01.30.702967

Figure Lengend Snippet: BrdU incorporation assay was used to measure cell proliferation. Quantification shows the percentage of BrdU-labeled cells in control (siC) and si5B-treated groups. Data are presented as mean ± SEM; ***p < 0.001. (A) UMSCC-29 (B) BJ-5ta (C) Collagen-based invasion assay was used to evaluate cell invasiveness in control (siC) and si5B-treated groups. Cells were seeded onto collagen matrices and allowed to invade for a defined period (48-72 hours). The number of invading cells was quantified and expressed as the mean ± SEM. Microscopy images of invading cells (C; left panel) , quantification (C; right panel) . Phase contrast microscopy images showing wound area in siC/si5B-treated OSCC cells at the time of scratch introduction (T = 0 h) & at T = 30 h wound closure (c; top panel) . Quantification of the percentage of wound area covered after 30 hours, normalized to wound area at the time of scratch introduction (c; lower panel) . (E) Representative images of the endothelial HUVEC cells tube formation assay treated with conditioned media from siC- or si5B-transfected OSCC. ImageJ angiogenesis macro software quantified Junctions, segments, and nodes. OSCC cells were transfected with either control siRNA (siC) or eIF5B-specific siRNA (si5B) and incubated for 96 hours. (F) The conditioned media for the control and eIF5B-depleted OSCC cells were analyzed for angiogenic biomarkers (lower panel) using EVE Technologies. (G) Representative images of immunoblots quantified probing for eIF5B, p-EGFR, EGFR, p-ERK, ERK, p-NF-kB, NF-kB, HIF1α, VEGFA, and β-actin (internal control). Data are presented as mean ± SEM from three independent biological replicates, with statistical significance indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001, and ****, p< 0.0001.

Article Snippet: CAL-33 and BJ-5ta (immortalized fibroblast) cell lines were obtained from American Type Culture Collection (ATCC; Virginia, USA), and UMSCC-1 and UMSCC-29 were obtained from the University of Michigan (Michigan, USA).

Techniques: BrdU Incorporation Assay, Labeling, Control, Invasion Assay, Microscopy, Tube Formation Assay, Transfection, Software, Incubation, Western Blot

Journal: bioRxiv

Article Title: Eukaryotic Initiation Factor 5B (eIF5B)-Driven Translational Control Impacts Oral Squamous Cell Carcinoma Pathophysiology

doi: 10.64898/2026.01.30.702967

Figure Lengend Snippet: BJ-5ta cells were transfected with either control siRNA (siC) or eIF5B-specific siRNA (si5B) and incubated for 96 hours. TRAIL (100 ng/mL) treatment was performed for 4 hours, then the media was collected. The conditioned media for the control and eIF5B-depleted BT5Ta cells were analyzed for angiogenic biomarkers using EVE Technologies. The level of distinct angiogenic biomarkers was decreased in the spent media of eIF5B-depleted BJ-5ta cells. Data are expressed as mean ± SEM for three biological replicates. Statistical significance is indicated as *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: CAL-33 and BJ-5ta (immortalized fibroblast) cell lines were obtained from American Type Culture Collection (ATCC; Virginia, USA), and UMSCC-1 and UMSCC-29 were obtained from the University of Michigan (Michigan, USA).

Techniques: Transfection, Control, Incubation